Welcome to the Galaxy Training Video Library!

This page shows you the full library of available GTN video tutorials, you can use these for self-study, courses, and more! Below are all of the individual videos, but some videos are part of multi-video “sessions” which can be found in the session library

Multi-Video Sessions Training Modules Adding Videos


All Training Videos

Here is the full list of all individual training videos

Admin

Tutorial Video

Length: 2 hours 50 minutes
Captions: Nate Coraor, Helena Rasche
Created: 1 March 2022
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Have an understanding of how Galaxy's Ansible roles are structured and interact with one another
  • Be able to use an Ansible playbook to install different flavors of Galaxy for different purposes
Go to the video page to see all versions.

Speakers

Lecture Video

Length: 5 minutes
Captions: Martin Čech
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
  • Learn Ansible basics
  • Write a simple role
  • Install a role from Ansible Galaxy
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 61 minutes
Captions: Martin Čech
Created: 15 February 2021
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Learn Ansible basics
  • Write a simple role
  • Install a role from Ansible Galaxy
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 31 minutes
Captions: Catherine Bromhead, Saskia Hiltemann
Created: 15 February 2021
Materials:
Support:
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 3 minutes
Captions: The Global Galaxy Community
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
  • Have an understanding of what CVMFS is and how it works
  • Install and configure the CVMFS client on a linux machine and mount the Galaxy reference data repository
  • Configure your Galaxy to use these reference genomes and indices
  • Use an Ansible playbook for all of the above.
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 23 minutes
Captions: Simon Gladman
Created: 15 February 2021
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Have an understanding of what CVMFS is and how it works
  • Install and configure the CVMFS client on a linux machine and mount the Galaxy reference data repository
  • Configure your Galaxy to use these reference genomes and indices
  • Use an Ansible playbook for all of the above.
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 22 minutes
Captions: Helena Rasche, Saskia Hiltemann
Created: 15 February 2021
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Setup a data library
  • Manage permissions
  • Import data from disk
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 2 minutes
Captions: The Global Galaxy Community
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
  • Learn gxadmin basics
  • See some queries and learn how they help debug production issues
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 44 minutes
Captions: Anthony Bretaudeau, Helena Rasche
Created: 15 February 2021
Materials:
Support:
Supported Servers: Possibly Working
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 31 minutes
Captions: Saskia Hiltemann
Created: 15 February 2021
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Know how to map tools to job destinations
  • Be able to use the dynamic job runner to make arbitrary destination mappings
  • Understand the job resource selector config and dynamic rule creation
  • The various ways in which tools can be mapped to destinations, both statically and dynamically
  • How to write a dynamic tool destination (DTD)
  • How to write a dynamic python function destination
  • How to use the job resource parameter selection feature
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 6 minutes
Captions: Beatriz Serrano-Solano
Created: 15 February 2021
Materials:
Support:
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Speaker

Lecture Video

Length: 2 minutes
Captions: The Global Galaxy Community
Created: 15 February 2021
Materials:
Support:
Go to the video page to see all versions.

Speaker

Lecture Video

Length: 2 minutes
Captions: The Global Galaxy Community
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
  • Setup InfluxDB
  • Setup Telegraf
  • Setup Grafana
  • Create several charts
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 73 minutes
Captions: Saskia Hiltemann, Helena Rasche
Created: 15 February 2021
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Setup InfluxDB
  • Setup Telegraf
  • Setup Grafana
  • Create several charts
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 18 minutes
Captions: Catherine Bromhead
Created: 15 February 2021
Materials:
Support:
Go to the video page to see all versions.

Speaker

Lecture Video

Length: 14 minutes
Captions: Saskia Hiltemann
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
  • Have an understanding of what Pulsar is and how it works
  • Install and configure a RabbitMQ message queueing server
  • Install and configure a Pulsar server on a remote linux machine
  • Be able to get Galaxy to send jobs to a remote Pulsar server
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 1 hour 28 minutes
Captions: Helena Rasche
Created: 28 June 2021
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Have an understanding of what Pulsar is and how it works
  • Install and configure a RabbitMQ message queueing server
  • Install and configure a Pulsar server on a remote linux machine
  • Be able to get Galaxy to send jobs to a remote Pulsar server
Go to the video page to see all versions.

Speaker

Tutorial Video

Description:

Running jobs in Apptainer
Length: 43 minutes
Captions: Cristóbal Gallardo, Simon Gladman
Created: 28 June 2021
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Configure your Galaxy to use Apptainer and BioContainers for running jobs
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 24 minutes
Captions: Helena Rasche, Saskia Hiltemann
Created: 15 February 2021
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Setup TIaaS
  • Request and manage trainings
  • Join a training
Go to the video page to see all versions.

Speaker

Lecture Video

Length: 17 minutes
Captions: Catherine Bromhead
Created: 28 June 2021
Materials:
Support:
Learning Objectives:
  • Learn about Ephemeris
  • Extract a list of tools from a workflow
  • Install these tools on a given Galaxy
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 27 minutes
Captions: Catherine Bromhead
Created: 28 June 2021
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Learn about Ephemeris
  • Extract a list of tools from a workflow
  • Install these tools on a given Galaxy
Go to the video page to see all versions.

Speaker

Tutorial Video

Description:

Here you’ll learn to setup TUS an open source resumable file upload server to process uploads for Galaxy. We use an external process here to offload the main Galaxy processes for more important work and not impact the entire system during periods of heavy uploading.
Length: 7 minutes
Captions: Helena Rasche
Created: 11 March 2022
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Setup TUSd
  • Configure Galaxy to use it to process uploads
Go to the video page to see all versions.

Speaker

Tutorial Video

Length: 36 minutes
Captions: Simon Gladman, Helena Rasche
Created: 15 February 2021
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Understand how to find the current Galaxy server version you are using
  • How to find the latest release version
  • How to backup the Galaxy server database and why it's a good idea
  • How to upgrade the Galaxy server to a new version
Go to the video page to see all versions.

Speaker

Lecture Video

Length: 4 minutes
Captions: The Global Galaxy Community
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
  • Learn the Galaxy user/group management and assign Quotas.
  • Understand the Role Based Access Control (RBAC) of Galaxy.
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Speaker

Tutorial Video

Length: 4 minutes
Captions: Saskia Hiltemann
Created: 15 February 2021
Materials:
Support:
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Speaker

Lecture Video

Description:

This supplementary welcome will give you additional details that are needed for the week.

Please note that when you use the mentioned /completed command, you do not need to specify a history URL. Just /completed is sufficient.
Length: 4 minutes
Captions: Helena Rasche
Created: 15 February 2021
Support:
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Speakers

Assembly

Tutorial Video

Description:

This tutorial guides you through the preprocessing of sequencing data of bronchoalveolar lavage fluid (BALF) samples obtained from early COVID-19 patients in China. Since such samples are expected to be contaminated signficantly with human sequenced reads, the goal is to enrich the data for SARS-CoV-2 reads by identifying and discarding reads of human origin before trying to assemble the viral genome sequence.
Length: 25 minutes
Captions: Mehmet Tekman
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Go to the video page to see all versions.

Speaker

Tutorial Video

Description:

In this tutorial, we will walk you through an assembly of a large genome, such as a plant or animal genome.
Length: 25 minutes
Captions: Anna Syme
Created: 16 May 2023
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Assemble a large genome from long reads
  • Polish the assembly
  • Assess the quality of the assembly
Go to the video page to see all versions.

Speaker

Lecture & Tutorial Video

Description:

In this tutorial, you will assemble a chloroplast genome using a combination of short and long read data.
Length: 35 minutes
Captions: Anna Syme
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Assemble a chloroplast genome from long reads
  • Polish the assembly with short reads
  • Annotate the assembly and view
  • Map reads to the assembly and view
Go to the video page to see all versions.

Speaker

Lecture Video

Description:

This lecture walks you through the basic concepts involved in assembling a genome from short-read sequencing data.
Length: 25 minutes
Captions: Saskia Hiltemann, Helena Rasche
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
  • assemble some paired end reads using Velvet
  • examine the output of the assembly.
Go to the video page to see all versions.

Speaker

Demo Video

Description:

This demo walks you through running the VGP Genome Assembly workflows, rather than the whole tutorial (as most GTN videos do)
Length: 10 minutes
Captions: Delphine Lariviere
Created: 13 March 2022
Materials:
Support:
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Speaker

Video

Description:

In this tutorial, we will learn how to run metagenomic assembly tool and evaluate the quality of the generated assemblies. To do that, we will use data from the study: Temporal shotgun metagenomic dissection of the coffee fermentation ecosystem. For an in-depth analysis of the structure and functions of the coffee microbiome, a temporal shotgun metagenomic study (six time points) was performed. The six samples have been sequenced with Illumina MiSeq utilizing whole genome sequencing.
Length: 1 hour
Captions: Bérénice Batut, Sophia Hampe
Created: 2 May 2023
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Describe what an assembly is
  • Describe what de-replication is
  • Explain the difference between co-assembly and individual assembly
  • Explain the difference between reads, contigs and scaffolds
  • Explain how tools based on De Bruijn graph work
  • Apply appropriate tools for analyzing the quality of metagenomic data
  • Construct and apply simple assembly pipelines on short read data
  • Apply appropriate tools for analyzing the quality of metagenomic assembly
  • Evaluate the Quality of the Assembly with Quast, Bowtie2, and CoverM-Genome
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Speaker

Climate

Lecture Video

Description:

This lecture provides a brief overview of FATES
Length: 7 minutes
Captions: Anne Fouilloux
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
  • Setting up a CLM-FATES case.
  • Customizing your run.
  • Interactive visualization with Panoply.
  • Automating your analyzes and visualisations of your CLM-FATES case.
  • Creating multi-case scenarios.
  • Composing, executing and publishing CML-FATES workflow.
Go to the video page to see all versions.

Speaker

Tutorial Video

Description:

This tutorial will walk you through a Galaxy analysis step by step
Length: 1 hour 20 minutes
Captions: Sébastien Fouilloux
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working
Learning Objectives:
  • Setting up a CLM-FATES case.
  • Customizing your run.
  • Interactive visualization with Panoply.
  • Automating your analyzes and visualisations of your CLM-FATES case.
  • Creating multi-case scenarios.
  • Composing, executing and publishing CML-FATES workflow.
Go to the video page to see all versions.

Speaker

Tutorial Video

Description:

In this tutorial, we will learn about Xarray, one of the most used Python library from the Pangeo ecosystem.

We will be using data from Copernicus Atmosphere Monitoring Service and more precisely PM2.5 (Particle Matter < 2.5 μm) 4 days forecast from December, 22 2021. Parallel data analysis with Pangeo is not covered in this tutorial.
Length: 17 minutes
Captions: Anne Fouilloux
Created: 3 March 2022
Materials:
Support:
Supported Servers: Known Working
Learning Objectives:
  • Learn to get metadata information using Xarray Galaxy Tools
  • Learn to select data
  • Learn to visualize data
  • Learn to filter, make reduction operations (mean, max, min)
  • Learn to resample my data
  • Learn to cite and contribute to Pangeo
Go to the video page to see all versions.

Speaker

Tutorial Video

Description:

In this tutorial, you will learn how to manipulate netCDF data files using Xarray Galaxy Tools. NetCDF stands for network Common Data Form and is one of the most popular file format in climate science. It is used for storing multidimensional scientific data variables such as temperature or humidity, and metadata can be added to facilitate sharing of netCDF data. netCDF is widely used outside the Climate Science community and each community has its own set of conventions, especially for metadata. The Climate and Forecast metadata convention, also called CF-convention is used by the Climate community and is designed to promote the processing and sharing of netCDF files.
Length: 1 hour 20 minutes
Captions: Anne Fouilloux
Created: 3 March 2022
Materials:
Support:
Supported Servers: Known Working
Learning Objectives:
  • Understand what Pangeo and Xarray are
  • Learn to get metadata information using Xarray Galaxy Tools
  • Learn to select data
  • Learn to visualize geographical data on a map
  • Learn to filter, make reduction operations (mean, max, min)
  • Learn to resample my data
Go to the video page to see all versions.

Speaker

Community

Welcome Video

Description:

This short video gives an overview of the worldwide Galaxy community, and different ways you can get involved! Video created by Beatriz Serrano-Solano.
Length: 5 minutes
Captions: Saskia Hiltemann
Created: 15 February 2021
Materials:
Support:
Go to the video page to see all versions.

Speaker

Computational chemistry

Tutorial Video

Description:

Molecular dynamics (MD) is a method to simulate molecular motion by iterative application of Newton’s laws of motion. This tutorial provides an introduction to using high-throughput molecular dynamics to study protein-ligand interaction, as applied to the N-terminal domain of Hsp90 (heat shock protein 90).
Length: 1 hour 40 minutes
Captions: Simon Bray
Created: 15 February 2021
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Learn about force-fields and MD parameterization
  • Learn how to conduct MD simulation and analysis for a protein-ligand system
  • Understand how different molecular interactions contribute to the binding affinity of various ligands for the Hsp90 protein.
Go to the video page to see all versions.

Speakers

Contributing

Lecture Video

Description:

This lecture goes through the process of how to create a slide deck, common things you should bear in mind while you’re writing them, and how to enable the amazing slides-to-video features for your slide decks! This video itself was produced automatically from the GTN’s slide decks, anyone making edits to these slides will cause them to be rebuilt, making organising videos for events easier than ever.
Length: 5 minutes
Captions: The Global Galaxy Community
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
  • Create a new set of slides
  • Add presenter comments
Go to the video page to see all versions.

Speaker

Tutorial Video

Description:

This tutorial will go through all of the steps of creating a simple tutorial and contributing it to the GTN.
Length: 42 minutes
Captions: Engy Nasr
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working
Learning Objectives:
  • Create a tutorial from scratch
  • Link a tutorial to a topic
  • Create hands-on
  • Add technical support for a tutorial
Go to the video page to see all versions.

Speaker

Tutorial Video

Description:

This tutorial will show you how you can preview your contributions to the GTN online using GitPod.
Length: 20 minutes
Captions: Saskia Hiltemann
Created: 15 February 2021
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Preview the GTN website online via GitPod
  • Make changes to the GTN website and preview those changes
Go to the video page to see all versions.

Speaker

Event

Tutorial Video

Length: 9 minutes
Captions: Helena Rasche
Created: 15 February 2021
Support:
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Speaker

Lecture Video

Description:

Start here; we will go over all the important things to know to get the most out of this workshop
Length: 15 minutes
Captions: Saskia Hiltemann, Helena Rasche
Created: 15 February 2021
Materials:
Support:
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Speakers

Welcome Video

Description:

Start here! We will go over all the important things you need to know about this week!
Length: 15 minutes
Captions: Saskia Hiltemann
Created: 10 March 2022
Materials:
Support:
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Speakers

Welcome Video

Description:

Start here! We will go over all the important things you need to know about this week!
Length: 15 minutes
Captions: Saskia Hiltemann
Created: 16 May 2023
Materials:
Support:
Go to the video page to see all versions.

Speakers

Data science

Tutorial Video

Description:

An advanced tutorial covering downstream analysis of RNA-seq data using R.
Length: 1 hour
Captions: Keith Suderman, Nadia Goué
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working
Learning Objectives:
  • Be able to load and explore the shape and contents of a tabular dataset using base R functions.
  • Understand factors and how they can be used to store and work with categorical data.
  • Apply common `dplyr` functions to manipulate data in R.
  • Employ the ‘pipe’ operator to link together a sequence of functions.
Go to the video page to see all versions.

Speaker

Tutorial Video

Description:

This tutorial will provide an introduction to using R with Rstudio in Galaxy.
Length: 50 minutes
Captions: Sébastien Fouilloux, Anne Fouilloux, Kaivan Kamali
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working
Learning Objectives:
  • Know advantages of analyzing data using R within Galaxy.
  • Compose an R script file containing comments, commands, objects, and functions.
  • Be able to work with objects (i.e. applying mathematical and logical operators, subsetting, retrieving values, etc).
Go to the video page to see all versions.

Speaker

Galaxy interface

Demo Video

Description:

This tutorial will show you how you can start Rstudio from within Galaxy. This option is only available on Galaxy EU for the time being. If you are working on a different Galaxy server, you can use Rstudio Cloud (https://rstudio.cloud/)
Length: 20 minutes
Captions: Saskia Hiltemann
Created: 15 February 2021
Materials:
Support:
Go to the video page to see all versions.

Speaker

Demo Video

Length: 18 minutes
Captions: Saskia Hiltemann
Created: 19 May 2023
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Gain understanding on navigating and manipulating histories
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Speaker

Tutorial Video

Length: 13 minutes
Captions: Delphine Lariviere
Created: 9 May 2023
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Understand and master dataset collections
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Speaker

Lecture Video

Description:

This lecture will introduce the SRA Aligned Read format available in the cloud from SRA, as well as some accompanying metadata that can help you search and filter the data. This sessions is aimed specifically at SARS-CoV-2 runs in SRA.
Length: 15 minutes
Captions: Jonathan Trow
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
  • Learn about SRA aligned read format and vcf files for Runs containing SARS-CoV-2 content
  • Understand how to search the metadata for these Runs to find your dataset of interest and then import that data in your preferred format
Go to the video page to see all versions.

Speaker

Tutorial Video

Description:

This tutorial will walk you through accessing and using SRA Aligned read format in Galaxy.
Length: 40 minutes
Captions: Jonathan Trow
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Learn about SRA aligned read format and vcf files for Runs containing SARS-CoV-2 content
  • Understand how to search the metadata for these Runs to find your dataset of interest and then import that data in your preferred format
Go to the video page to see all versions.

Speaker

Tutorial Video

Description:

Through a series of examples, this tutorial aims to familiarize the reader with building Galaxy collections from tabular data containing URLs, sample sheets, list of accessions or identifiers, etc..
Length: 22 minutes
Captions: Assunta DeSanto
Created: 15 February 2021
Materials:
Support:
Supported Servers: Possibly Working
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Speaker

Lecture & Tutorial Video

Description:

So you’ve used Galaxy workflows to analyze your SARS-CoV-2 samples? Learn in this tutorial how to export results to your favorite datastore.
Length: 10 minutes
Captions: Helena Rasche
Created: 9 August 2021
Support:
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Speaker

Tutorial Video

Description:

This is a more technical tutorial which will teach you how to run workflows from the command line and scale your analyses. If you are on Windows, you will need WSL/WSL2 setup before this session.
Length: 30 minutes
Captions: Simon Bray
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
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Speaker

Tutorial Video

Description:

To make a workflow reusable with slightly different settings you can define and use workflow parameters.
Length: 30 minutes
Captions: Marius van den Beek
Created: 6 July 2022
Materials:
Support:
Supported Servers: Known Working
Learning Objectives:
  • Learn how to use Workflow Parameters to improve your Workflows
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Speaker

Tutorial Video

Description:

Learn how to view, customize and share Galaxy workflow reports
Length: 30 minutes
Captions: Saskia Hiltemann
Created: 6 July 2022
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Understanding, viewing and creating workflow reports
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Speaker

Dev

Lecture Video

Description:

TODO
Length: 15 minutes
Captions: No captions available for this video. We aim to have captions for all our videos, and are working to add captions to this video as soon as possible.
Created: 15 February 2021
Materials:
Support:
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Speaker

Lecture Video

Description:

TODO
Length: 25 minutes
Captions: No captions available for this video. We aim to have captions for all our videos, and are working to add captions to this video as soon as possible.
Created: 15 February 2021
Materials:
Support:
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Speaker

Lecture Series Video

Description:

TODO
Length: 17 minutes
Captions: No captions available for this video. We aim to have captions for all our videos, and are working to add captions to this video as soon as possible.
Created: 15 February 2021
Materials:
Support:
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Speaker

Lecture Video

Description:

TODO
Length: 15 minutes
Captions: No captions available for this video. We aim to have captions for all our videos, and are working to add captions to this video as soon as possible.
Created: 15 February 2021
Materials:
Support:
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Speaker

Lecture Video

Description:

TODO
Length: 45 minutes
Captions: No captions available for this video. We aim to have captions for all our videos, and are working to add captions to this video as soon as possible.
Created: 15 February 2021
Materials:
Support:
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Speaker

Lecture Video

Description:

TODO
Length: 5 minutes
Captions: Saskia Hiltemann
Created: 15 February 2021
Materials:
Support:
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Speaker

Lecture Video

Description:

TODO
Length: 25 minutes
Captions: Mallory Freeberg
Created: 15 February 2021
Materials:
Support:
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Speaker

Lecture Video

Description:

TODO
Length: 15 minutes
Captions: No captions available for this video. We aim to have captions for all our videos, and are working to add captions to this video as soon as possible.
Created: 15 February 2021
Materials:
Support:
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Speaker

Lecture Video

Description:

TODO
Length: 35 minutes
Captions: No captions available for this video. We aim to have captions for all our videos, and are working to add captions to this video as soon as possible.
Created: 15 February 2021
Materials:
Support:
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Speaker

Tutorial Video

Description:

What to do when an API test fails.
Length: 23 minutes
Captions: John Davis
Created: 15 February 2021
Materials:
Support:
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Speaker

Tutorial Video

Description:

This video covers working through the client linting error hit in the previous tutorial.
Length: 6 minutes
Captions: Beatriz Serrano-Solano
Created: 15 February 2021
Materials:
Support:
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Speaker

Tutorial Video

Description:

What to do when your client throws a unit test failure.
Length: 6 minutes
Captions: Beatriz Serrano-Solano
Created: 15 February 2021
Materials:
Support:
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Speaker

Tutorial Video

Description:

This is the first video in the Debugging Galaxy series.
Length: 11 minutes
Captions: Dave Clements
Created: 15 February 2021
Materials:
Support:
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Speakers

Tutorial Video

Description:

How to fix a runtime error
Length: 40 minutes
Captions: John Davis
Created: 15 February 2021
Materials:
Support:
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Speaker

Tutorial Video

Description:

Selenium is used to test Galaxy development. Learn more about test failure and how to address them.
Length: 18 minutes
Captions: Assunta DeSanto, John Davis, Dave Clements, Beatriz Serrano-Solano
Created: 15 February 2021
Materials:
Support:
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Speaker

Lecture Video

Description:

A summary of wisdom to debug Galaxy with.
Length: 3 minutes
Captions: Dave Clements
Created: 15 February 2021
Materials:
Support:
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Speakers

Tutorial Video

Description:

What to do when a unit test fails.
Length: 14 minutes
Captions: John Davis
Created: 15 February 2021
Materials:
Support:
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Speaker

Tutorial Video

Description:
  1. The first step to adding a tool to Galaxy, is to add it to (Bio)Conda. This tutorial will show you how to do that.
  2. Planemo is the Galaxy tool development kit, and will make your tool development process a lot easier! This tutorial will show you how to use Planemo to start the tool wrapping process, and covers the general structure of a Galaxy tool wrapper.
  3. You are now ready to start writing your tool wrapper! This video will show you how.
  4. Each tool wrapper also needs a .shed.yml file, which contains some metadata about the tool.
  5. Here you will learn how to test Galaxy tools using Planemo.
  6. Here you will learn how to publish tools in public tool repositories.
Length: 50 minutes
Captions: Cristóbal Gallardo, Alex Ostrovsky
Created: 18 May 2023
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Learn how to make and deploy a conda package
  • Understand how to write a Galaxy tool wrapper
  • Be able to use a tool wrapper locally
  • Be able to test the tool wrapper
  • Learn how to add tools to the Galaxy toolshed and make them available to any user
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Speakers

Video

Description:

Hands on demonstration for the Introductory tutorial. Create, run and update a “Hello World” tool in Galaxy
Length: 7 minutes
Captions: Ross Lazarus
Created: 15 February 2021
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Further develop your ToolFactory Skills
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Speaker

Video

Description:

ToolFactory Presentation: Abstract tool interface code that wraps simple scripts can be generated using a Galaxy tool. If you haven’t already decided that the GTN tutorials linked below might be useful for your work, or if want to hear Ross’s opinions on tools, abstract interfaces and why they matter, this talk is for you.
Length: 30 minutes
Captions: Ross Lazarus
Created: 15 February 2021
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Learn why you might want to use the ToolFactory Appliance
  • Watch a video demonstration and explore the generated code - Hello Galaxy Training Network!
  • Run it locally in Docker.
  • Install and explore the simple examples provided.
  • Modify and re-generate them to see how the changes affect the tool
  • Generate new simple Galaxy tools using your own scripts
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Speaker

Ecology

Lecture Video

Description:

Based on this OBITools official tutorial, you will learn here how to analyze DNA metabarcoding data produced on Illumina sequencers
Length: 1 hour 15 minutes
Captions: Yvan Le Bras
Created: 3 March 2022
Materials:
Support:
Supported Servers: Known Working
Learning Objectives:
  • Deal with paired-end data to create consensus sequences
  • Clean, filter and anlayse data to obtain strong results
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Speaker

Lecture Video

Description:

This tutorial aims to present the PAMPA Galaxy workflow, how to use it to compute common biodiversity metrics from species abundance data and analyse it through generalized linear (mixed) models (GLM and GLMM). This workflow made up of 5 tools will allow you to process temporal series data that include at least year, location and species sampled along with abundance value and, finally, generate article-ready data products.
Length: 2 hours
Captions: Coline Royaux
Created: 3 March 2022
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Upload data from DATRAS portal of ICES
  • Pre-process population data with Galaxy
  • Learning how to use an ecological analysis workflow from raw data to graphical representations
  • Learning how to construct a Generalized Linear (Mixed) Model from a usual ecological question
  • Learning how to interpret a Generalized Linear (Mixed) Model
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Speaker

Lecture Video

Length: 1 hour
Captions: Olivier Norvez
Created: 3 March 2022
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Explore Biodiversity data with taxonomic, temporal and geographical informations
  • Have an idea about quality content of the data regarding statistical tests like normality or homoscedasticity and coverage like temporal or geographical coverage
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Speaker

Epigenetics

Lecture Video

Description:

This lecture will introduce the basic concepts involved in ATAC-Seq analysis.
Length: 15 minutes
Captions: Lucille Delisle
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
  • Apply appropriate analysis and quality control steps for ATAC-Seq
  • Generate a heatmap of transcription start site accessibility
  • Visualise peaks for specific regions
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Speaker

Tutorial Video

Description:

This tutorial will walk you through a ATAC-Seq data analysis.
Length: 1 hour 50 minutes
Captions: Hans-Rudolf Hotz
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Apply appropriate analysis and quality control steps for ATAC-Seq
  • Generate a heatmap of transcription start site accessibility
  • Visualise peaks for specific regions
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Speaker

Galaxy intro

Demo Video

Description:

This demo videos shows you how you can run Interactive tools such as RStudio and Jupyter notebooks within Galaxy.
Length: 15 minutes
Captions: Saskia Hiltemann
Created: 7 July 2022
Support:
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Speaker

Lecture & Demo Video

Description:

This video will introduce the Galaxy data analysis platform, and give a short demo on how to use it.
Length: 10 minutes
Captions: Anton Nekrutenko
Created: 15 February 2021
Materials:
Support:
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Speaker

Tutorial Video

Description:

In this tutorial, we will walk you through your first (toy) analysis in Galaxy. This tutorial is aimed at familiarizing you with the Galaxy platform, and some basic NGS concepts.
Length: 1 hour 30 minutes
Captions: Natalie Kucher
Created: 18 May 2023
Materials:
Support:
Supported Servers: Known Working Possibly Working
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Speaker

Galaxy

Demo Video

Description:

This demo videos shows you how you can run Interactive tools such as RStudio and Jupyter notebooks within Galaxy.
Length: 15 minutes
Captions: Saskia Hiltemann
Created: 7 July 2022
Support:
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Speaker

Lecture & Demo Video

Description:

This video will introduce the Galaxy data analysis platform, and give a short demo on how to use it.
Length: 10 minutes
Captions: Anton Nekrutenko
Created: 15 February 2021
Materials:
Support:
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Speaker

Tutorial Video

Description:

In this tutorial, we will walk you through your first (toy) analysis in Galaxy. This tutorial is aimed at familiarizing you with the Galaxy platform, and some basic NGS concepts.
Length: 1 hour 30 minutes
Captions: Natalie Kucher
Created: 18 May 2023
Materials:
Support:
Supported Servers: Known Working Possibly Working
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Speaker

Tutorial Video

Description:

This video tutorial will provide a non-genomics based first look at the Galaxy platform, how to use it, and discusses how you can get support for your data analysis.
Length: 20 minutes
Captions: Alex Ostrovsky
Created: 1 May 2023
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Familiarize yourself with the basics of Galaxy
  • Learn how to obtain data from external sources
  • Learn how to tag datasets
  • Learn how to run tools
  • Learn how histories work
  • Learn how to create a workflow
  • Learn how to share your work
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Speaker

Sars cov2

Lecture & Tutorial Video

Description:

So you’ve used Galaxy workflows to analyze your SARS-CoV-2 samples? Learn in this tutorial how to export results to your favorite datastore.
Length: 10 minutes
Captions: Helena Rasche
Created: 9 August 2021
Support:
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Speaker

Demo Video

Description:

In this demo you will get to know the ObservableHQ platform for interactive data visualization. You will see how covid19.galaxyproject.org uses it to build a dashboard for their SARS-CoV-2 analysis efforts and will learn how to customize this solution to fit your own purposes.
Length: 15 minutes
Captions: Assunta DeSanto
Created: 8 December 2021
Support:
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Speaker

Demo Video

Length: 10 minutes
Captions: Pia H., Assunta DeSanto
Created: 8 December 2021
Support:
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Speaker

Demo Video

Description:

See in this demo how, on usegalaxy.*, we’ve used Planemo and Bioblend to build and operate an automated SARS-CoV-2 genome surveillance system based on the Galaxy workflows for variant calling, consensus building and reporting.
Length: 40 minutes
Captions: Helena Rasche
Created: 9 August 2021
Support:
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Speaker

Tutorial Video

Description:

Get an overview of the workshop: production-ready Galaxy workflows for SARS-CoV-2 sequencing data, tools you should know to automate workflow execution, and how you combine all of it to turn Galaxy into a platform for genome-surveillance
Length: 13 minutes
Captions: Pia H.
Created: 9 August 2021
Support:
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Speaker

Demo Video

Description:

How to visualize tens of thousands of SARS-CoV-2 analysis results? Learn about the Viral Beacon project’s solution!
Length: 24 minutes
Captions: Assunta DeSanto
Created: 8 December 2021
Materials:
Support:
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Speaker

Genome annotation

Lecture Video

Description:

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.
Length: 3 minutes
Captions: Anthony Bretaudeau
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
  • Load genome into Galaxy
  • Annotate genome with Prokka
  • View annotations in JBrowse
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Speaker

Tutorial Video

Description:

In this tutorial we will use Prokka to annotate a draft genome sequence. Prokka finds and annotates features (both protein coding regions and RNA genes, i.e. tRNA, rRNA) present on on a sequence. Prokka can be used to annotate bacterial, archaeal and viral genomes quickly, generating standard output files in GenBank, EMBL and gff formats.”
Length: 20 minutes
Captions: Anthony Bretaudeau
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Load genome into Galaxy
  • Annotate genome with Prokka
  • View annotations in JBrowse
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Speaker

Lecture Video

Description:

This lecture gives a quick overview of Apollo
Length: 5 minutes
Captions: Anthony Bretaudeau
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
  • Load a genome into Galaxy
  • View annotations in JBrowse
  • Learn how to load JBrowse data into Apollo
  • Learn how to manually refine genome annotations within Apollo
  • Export refined genome annotations
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Speaker

Tutorial Video

Description:

In this tutorial you will get some hands-on experience using Apollo in Galaxy
Length: 1 hour
Captions: Anthony Bretaudeau
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Load a genome into Galaxy
  • View annotations in JBrowse
  • Learn how to load JBrowse data into Apollo
  • Learn how to manually refine genome annotations within Apollo
  • Export refined genome annotations
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Speaker

Tutorial Video

Description:

Genome annotation of eukaryotes is a little more complicated than for prokaryotes: eukaryotic genomes are usually larger than prokaryotes, with more genes. The sequences determining the beginning and the end of a gene are generally less conserved than the prokaryotic ones. Many genes also contain introns, and the limits of these introns (acceptor and donor sites) are not highly conserved.

In this tutorial we will use a software tool called Funannotate (Palmer and Stajich 2020) to annotate the genome sequence of a small eukaryote: Mucor mucedo (a fungal plant pathogen).
Length: 1 hour 10 minutes
Captions: Anthony Bretaudeau
Created: 12 May 2023
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Load genome into Galaxy
  • Annotate genome with Funannotate
  • Perform functional annotation using EggNOG-mapper and InterProScan
  • Evaluate annotation quality with BUSCO
  • View annotations in JBrowse
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Speaker

Tutorial Video

Description:

The aim of repeat masking is to identify the location of all repeated elements along a genome sequence. Other tools (like annotation pipelines) can then take this information into account when producing their results.
Length: 18 minutes
Captions: Anthony Bretaudeau
Created: 10 May 2023
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Use Red and RepeatMasker to soft-mask a newly assembled genome
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Speaker

Lecture Video

Description:

This lecture talks about the basics of TnSeq analysis
Length: 10 minutes
Captions: No captions available for this video. We aim to have captions for all our videos, and are working to add captions to this video as soon as possible.
Created: 10 March 2022
Materials:
Support:
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Speaker

Crispr

Lecture Video

Description:

This lecture will introduce the basic concepts involved in CRISPR screen analysis.
Length: 3 minutes
Captions: Maria Doyle
Created: 10 March 2022
Materials:
Support:
Learning Objectives:
  • Apply appropriate analysis and quality control steps for CRISPR screen data
  • Identify differentially enriched genes across conditions
  • Generate volcano plot to visualise results
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Speaker

Tutorial Video

Description:

This tutorial will walk you through a CRISPR screen analysis.
Length: 45 minutes
Captions: Maria Doyle
Created: 10 March 2022
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Apply appropriate analysis and quality control steps for CRISPR screen data
  • Identify differentially enriched genes across conditions
  • Generate volcano plot to visualise results
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Speaker

Introduction

Tutorial Video

Description:

This tutorial will introduce you to Galaxy. You will familiarize yourself with tools, workflows and histories. Those skills will be needed the next days
Length: 13 minutes
Captions: Saskia Hiltemann
Created: 21 July 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Familiarize yourself with the basics of Galaxy
  • Learn how to obtain data from external sources
  • Learn how to run tools
  • Learn how histories work
  • Learn how to create a workflow
  • Learn how to share your work
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Speaker

Demo Video

Description:

In this demo video, we will show how to perform an NGS data analysis, using a SARS-CoV-2 example dataset. If you would like to run the full tutorial yourself, please find the link below (duration 1h-1.5h).
Length: 15 minutes
Captions: Anton Nekrutenko
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
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Speaker

Variant analysis

Demo Video

Description:

A demo of SARS-CoV-2 sequencing data analysis from sequenced reads in NCBI’s SRA to called and annotated mutations for a batch of viral samples.
Length: 15 minutes
Captions: Anton Nekrutenko
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
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Speaker

Lecture Video

Length: 12 minutes
Captions: Wolfgang Maier
Created: 4 May 2023
Support:
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Speaker

Tutorial Video

Length: 55 minutes
Captions: Wolfgang Maier
Created: 10 May 2023
Materials:
Support:
Supported Servers: Known Working
Learning Objectives:
  • Discover and obtain recommended Galaxy workflows for SARS-CoV-2 sequence data analysis through public workflow registries
  • Choose and run a workflow to discover mutations in a batch of viral samples from sequencing data obtained through a range of different protocols and platforms
  • Run a workflow to summarize and visualize the mutation discovery results for a batch of samples
  • Run a workflow to construct viral consensus sequences for the samples in a batch
  • Know different SARS-CoV-2 lineage classification systems, and use pangolin and Nextclade to assign samples to predefined lineages
  • Combine information from different analysis steps to be able to draw appropriate conclusions about individual samples and batches of viral data
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Speaker

Lecture Video

Description:

Tuberculosis (TB) is an infectious disease caused by the bacterium Mycobacterium tuberculosis. According to the WHO, in 2018 there were 10.0 million new cases of TB worldwide and 1.4 million deaths due to the disease, making TB the world’s most deadly infectious disease.
Length: 20 minutes
Captions: Saskia Hiltemann
Created: 15 February 2021
Materials:
Support:
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Speaker

Tutorial Video

Description:

Variation in the genome of M. tuberculosis (Mtb) is associated with changes in phenotype, for example drug resistance and virulence. It is also useful for outbreak investigation as the single nucleotide polymorphisms (SNPs) in a sample can be used to build a phylogeny.
Length: 40 minutes
Captions: Peter van Heusden, Nadia Goué, Helena Rasche
Created: 15 February 2021
Materials:
Support:
Supported Servers:
Learning Objectives:
  • How should we filter those variants
  • How can we predict drug resistance from those variants
  • How do we annotate those variants
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Speaker

Tutorial Video

Description:

To discover causal mutations of inherited diseases it’s common practice to do a trio analysis. In a trio analysis DNA is sequenced of both the patient and parents. Using this method, it’s possible to identify multiple inheritance patterns.
Length: 40 minutes
Captions: Jasper Ouwerkerk
Created: 12 May 2023
Materials:
Support:
Supported Servers: Known Working
Learning Objectives:
  • Requesting DAC access and importing data from the EGA.
  • Pre-process VCFs using regular expressions.
  • Use annotations and phenotype information to find the causative variant(s).
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Speaker

Metagenomics

Lecture Video

Description:

Nanopore Sequencing has many applications in a clinical setting. In this lecture, Astrid Heikema discusses how her group at the Erasmus Medical Center uses Nanopore for sequencing of bacterial Genomes.
Length: 30 minutes
Captions: Helena Rasche
Created: 15 February 2021
Support:
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Speaker

Lecture Video

Description:

This lecture briefly introduces 16S sequencing, a popular technique used for taxonomic profiling of microbial communities.
Length: 15 minutes
Captions: Saskia Hiltemann
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
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Speaker

Tutorial Video

Description:

Metatranscriptomics analysis examines how the microbiome responds to the environment by studying the taxonomic composition and functional analysis of genes expressed by the microbiome, using microbial community RNASeq data and subsequent metatranscriptomics workflows. This workshop will introduce researchers to the basic concepts and tools from the ASaiM-MT workflow. ASaiM-MT provides a curated collection of tools to explore and visualize taxonomic and functional information from metatranscriptomic sequences.
Length: 1 hour 5 minutes
Captions: Paul Zierep
Created: 17 May 2023
Materials:
Support:
Supported Servers: Possibly Working
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Speaker

Video

Length: 1 hour 15 minutes
Captions: Sophia Hampe
Created: 16 May 2023
Materials:
Support:
Supported Servers: Known Working
Learning Objectives:
  • Explain what taxonomic assignment is
  • Explain how taxonomic assignment works
  • Apply Kraken and MetaPhlAn to assign taxonomic labels
  • Apply Krona and Pavian to visualize results of assignment and understand the output
  • Identify taxonomic classification tool that fits best depending on their data
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Speaker

Tutorial Video

Description:

This lecture briefly introduces 16S sequencing, a popular technique used for taxonomic profiling of microbial communities.
Length: 1 hour 37 minutes
Captions: Saskia Hiltemann
Created: 19 May 2023
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Analyze of 16S rRNA sequencing data using the mothur toolsuite in Galaxy
  • Using a mock community to assess the error rate of your sequencing experiment
  • Visualize sample diversity using Krona and Phinch
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Speaker

Tutorial Video

Description:

This tutorial will guide you through the process of assembling your Nanopore-sequenced bacterial samples and determining the presence of antibiotic resistance genes.
Length: 45 minutes
Captions: Bérénice Batut
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Perform Quality control on your reads
  • Assemble a genome with Minimap2/Miniasm/Racon
  • Determine the structure of the genome(s)
  • Scan for antimicrobial resistance genes with Staramr
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Speaker

Video

Description:

In this tutorial, we will be presenting a series of Galaxy workflows whose main goals are 1) to agnostically detect pathogens (What exactly is this pathogen and what virulence factors does it carry?) from data extracted directly (without prior cultivation) from a potentially contaminated sample (e.g. food like chicken, beef, etc.) and sequenced using Nanopore, and 2) to compare different samples to trace the possible source of contamination
Length: 1 hour 45 minutes
Captions: Engy Nasr
Created: 1 May 2023
Materials:
Support:
Supported Servers: Known Working
Learning Objectives:
  • Check quality reports generated by FastQC and NanoPlot for metagenomics Nanopore data
  • Preprocess the sequencing data to remove adapters, poor quality base content and host/contaminating reads
  • Perform taxonomy profiling indicating and visualizing up to species level in the samples
  • Identify pathogens based on the found virulence factor gene products via assembly, identify strains and indicate all antimicrobial resistance genes in samples
  • Identify pathogens via SNP calling and build the consensus gemone of the samples
  • Relate all samples' pathogenic genes for tracking pathogens via phylogenetic trees and heatmaps
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Speaker

Tutorial Video

Length: 1 hour 5 minutes
Captions: Sophia Hampe
Created: 10 May 2023
Materials:
Support:
Supported Servers: Known Working
Learning Objectives:
  • Inspect metagenomics data
  • Run metagenomics tools
  • Identify yeast species contained in a sequenced beer sample using DNA
  • Visualize the microbiome community of a beer sample
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Speaker

Video

Description:

In this tutorial, we will learn how to run metagenomic assembly tool and evaluate the quality of the generated assemblies. To do that, we will use data from the study: Temporal shotgun metagenomic dissection of the coffee fermentation ecosystem. For an in-depth analysis of the structure and functions of the coffee microbiome, a temporal shotgun metagenomic study (six time points) was performed. The six samples have been sequenced with Illumina MiSeq utilizing whole genome sequencing.
Length: 1 hour
Captions: Bérénice Batut, Sophia Hampe
Created: 2 May 2023
Materials:
Support:
Supported Servers: Possibly Working
Learning Objectives:
  • Describe what an assembly is
  • Describe what de-replication is
  • Explain the difference between co-assembly and individual assembly
  • Explain the difference between reads, contigs and scaffolds
  • Explain how tools based on De Bruijn graph work
  • Apply appropriate tools for analyzing the quality of metagenomic data
  • Construct and apply simple assembly pipelines on short read data
  • Apply appropriate tools for analyzing the quality of metagenomic assembly
  • Evaluate the Quality of the Assembly with Quast, Bowtie2, and CoverM-Genome
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Speaker

Proteogenomics

Lecture Video

Description:

In this opening presentation, the basic components of proteogenomics are described, including the main steps in the bioinformatics analysis workflow that make up this approach and will be detailed in the following tutorials. Some examples of research questions that benefit from a proteogenomics approach are also highlighted.
Length: 20 minutes
Captions: Emma Leith
Created: 15 February 2021
Materials:
Support:
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Speaker

Proteomics

Lecture Video

Description:

This lecture will introduce EncyclopeDIA.
Length: 20 minutes
Captions: Emma Leith
Created: 25 June 2021
Materials:
Support:
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Speakers

Tutorial Video

Description:

This tutorial will walk you through an analysis with EncyclopeDIA step by step.
Length: 30 minutes
Captions: Mehmet Tekman
Created: 25 June 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Understand the difference between DDA and DIA methods
  • Performing quantitative analysis using DIA data
  • Understand the purpose and benefits of using a Chromatogram Library for detection of peptides
  • You can use Bloom's Taxonomy to write effective learning objectives
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Speaker

Lecture Video

Description:

This lecture will provide an introduction to mass spectrometry (MS) based proteomics analysis. Slides created by Melanie Föll.
Length: 20 minutes
Captions: Melanie Föll
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
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Speaker

Tutorial Video

Description:

Modern mass spectrometry-based proteomics enables the identification and quantification of thousands of proteins. Therefore, quantitative mass spectrometry represents an indispensable technology for biological and clinical research. Statistical analyses are required for the unbiased answering of scientific questions and to uncover all important information in the proteomic data. In this training we will cover the full analysis workflow from label-free, data dependent acquisition (DDA) raw data to statistical results. We’ll use two popular quantitative proteomics software: MaxQuant and MSstats.
Length: 1 hour
Captions: Melanie Föll
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Learn how to use MaxQuant and MSstats for the analysis of label-free shotgun (DDA) data
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Speaker

Demo Video

Description:

In this tutorial, the basic workflow for metaproteomics is described, which includes database search, seeking taxonomy information and functional analysis. Related metaproteomics and functional microbiome tools and GTN tutorials are also mentioned in this tutorial.
Length: 1 hour
Captions: Emma Leith
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • A taxonomy and functional analysis of metaproteomic mass spectrometry data.
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Speaker

Lecture & Demo Video

Description:

The Coronavirus Disease 2019 (COVID-19) global pandemic has had a profound, lasting impact on the world’s population. Accurate and timely diagnosis of COVID-19 infection is an important step for providing care and containing its further spread. In this tutorial, attendees will be introduced to two workflows – a) database search workflow and b) peptide validation workflow. Attendees of the workshop will get an in-depth knowledge of the Galaxy workflows that detect SARS-CoV-2 peptides (10.1186/s12014-021-09321-1)) and co-infecting pathogen peptides (10.1021/acs.jproteome.0c00822).
Length: 35 minutes
Captions: Emma Leith
Created: 15 February 2021
Materials:
Support:
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Speakers

Tutorial Video

Description:

In this tutorial, we will provide a walkthrough on how to generate a customized protein sequence database using RNA-Seq data. The resultant FASTA database contains sequences with single amino acid variants (SAVs), insertions and deletions (indels)and transcript assemblies (splicing variants). This database can then be used to identify protein sequence variants from the mass spectrometry data.
Length: 40 minutes
Captions: Emma Leith
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Generating a customized Protein sequence database with variants, indels, splice junctions and known sequences.
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Speaker

Demo Video

Description:

In this demonstration, we’re continuing the process of proteogenomic analysis. Here, we search mass spectrometry data against a custom proteogenomics FASTA database to create peptide spectral matches (PSMs) for each dataset; we also isolate non-canonical peptides from the resulting data for further analysis. If you would like to view the results yourself, we provide example histories in the FAQ document
Length: 30 minutes
Captions: Emma Leith
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • A proteogenomic data analysis of mass spectrometry data to identify and visualize variant peptides.
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Speaker

Demo Video

Description:

In this demonstration, we will investigate the peptides that were identified during proteogenomics tutorial 2. We will identify the presence of novel proteoforms that are absent in the reference database, annotate the novel peptides and visualize them using the Multi-omics Visualization Platform (MVP). If you would like to view the results yourself, we provide example histories in the FAQ document
Length: 40 minutes
Captions: Subina Mehta
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • How to assign and visualize the genomic localization of these identified novel proteoforms?
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Speaker

Tutorial Video

Description:

This tutorial covers an annotation pipeline for a protein list identified by LC-MS/MS experiments.
Length: 30 minutes
Captions: Florence Combes
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working Possibly Working
Learning Objectives:
  • Execute a complete annotation pipeline of a protein list identified by LC-MS/MS experiments
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Speaker

Virology

Lecture Video

Length: 12 minutes
Captions: Wolfgang Maier
Created: 4 May 2023
Support:
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Speaker

One health

Lecture Video

Length: 10 minutes
Captions: Wolfgang Maier
Created: 19 May 2023
Materials:
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Speaker

Sequence analysis

Removal of human reads from SARS-CoV-2 sequencing data

image indicating this is a self-study session. This is a self-study session. Please work through the materials on your own, and ask the instructors for help if you get stuck or have any questions!
Tutorial: Removal of human reads from SARS-CoV-2 sequencing data
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Instructor

Lecture Video

Description:

This lecture covers the basic concepts involved in mapping sequencing reads to a reference genome.
Length: 10 minutes
Captions: Daniel Blankenberg
Created: 15 February 2021
Materials:
Support:
Learning Objectives:
  • You will learn what mapping is
  • A genome browser is shown that helps you to understand your data
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Speaker

Tutorial Video

Description:

In this tutorial you will map sequencing data to a reference genome, and explore the mapped reads in a genome browser.
Length: 20 minutes
Captions: Saskia Hiltemann
Created: 15 February 2021
Materials:
Support:
Supported Servers: Known Working