Warning: Please be aware that these videos are a snapshot, and as such may use an outdated version of the tutorial and/or Galaxy. Below the video you will find links to the tutorials as they appeared at the time of recording.
Bulk RNA Deconvolution with MuSiC
Below are video tutorials for this GTN material, created for various (past) events.
Tutorial Video (March 2021)
Description:
Bulk RNA-seq data contains a mixture of transcript signatures from
several types of cells. We wish to deconvolve this mixture to obtain
estimates of the proportions of cell types within the bulk sample. To
do this, we can use single cell RNA-seq data as a reference for
estimating the cell type proportions within the bulk data.
In this tutorial, we will use bulk and single-cell RNA-seq data, including
matrices of similar tissues from different sources, to illustrate how to infer
cell type abundances from bulk RNA-seq.