Module: Plant Transcriptomics
This module covers the contents of the plant transcriptome analysis using Galaxy workshop, first organized in 2021.
Introduction to NGS Data Analysis
In this sesssion, we will walk you through the basics of quality checking your sequencing data. This session consists of a lecture followed by a hands-on tutorial.
Quality Control (Lecture)
Quality Control (Tutorial)
Bulk RNA-Seq data analysis
In this sesssion, we will walk you through the basics of mapping your sequencing data to a reference genome. This session consists of a lecture followed by a hands-on tutorial.
In plants, miRNAs can silence targets through RNA degradation as well as translational repression pathways, and unlike animals, a large proportion of miRNA and their targets have less than four mismatches. This feature has been exploited for developing miRNAs target prediction tools, providing an efficient approach to elucidate the miRNA-mediated regulatory networks, which can contribute to biotechnological solutions to improve crops productivity.
Whole transcriptome analysis of Arabidopsis thaliana (Lecture)
Whole transcriptome analysis of Arabidopsis thaliana (Tutorial)
Intro to scRNA-seq analysis
Downstream scRNA-seq analysis